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KMID : 0380019950100050553
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 5 p.553 ~ p.559
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Antirbacterial Activity of Lactobacillus sp. KJ-5 Isolated from Pig Feces
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Abstract
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A lactic acid bacteria producing antibacterial substance was isolated from pig feces. This strain was idendified as a genus Lactobacillus, through its morphological, cultural and physiological characteristics. Lactobacillus sp. KJ-5 isolated showed the strong inhibitory effect on the growth of Salmonella paratyphi. The production of antibacterial substance was growth associated form during the batch culture of Lactobacillus sp. KJ-5 and the maximum production was obtained at the culture temperature of 371C as well as optimum temperature of cell growth. The antibacterial activity of the filtrate of culture broth was decreased by adjusting the pH 6.2 and was not affected by catalase treatment. The antibacterial substance was partially purified by methanol and acetone extraction, which exhibited three spots in the thin-layer chromatography and one of them showed an antibacterial activity. This substance also showed the maximum absorption of UV at 270nm and an antibacterial activity was completely inactivated by the treatment of proteolytic enzymes.
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